3Q6F
Crystal structure of Fab of human mAb 2909 specific for quaternary neutralizing epitope of HIV-1 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 178.504, 178.504, 218.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.653 - 3.192 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 66903 | |
| Completeness [%] | 99.5 | 78.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
| 1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
| 1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
| 1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
