3Q69
X-ray crystal structure of a MucBP domain of the protein LBA1460 from Lactobacillus acidophilus, Northeast structural genomics consortium target LaR80A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.789, 24.855, 83.363 |
| Unit cell angles | 90.00, 108.53, 90.00 |
Refinement procedure
| Resolution | 29.290 - 2.400 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | |
| Number of reflections | 9462 | |
| Completeness [%] | 98.0 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, .1M KBr .1M Bis Tris Propane pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
