3Q5Z
Crystal structure of virulent allele ROP5B pseudokinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.340, 70.553, 43.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.727 - 1.900 |
| R-factor | 0.2123 |
| Rwork | 0.209 |
| R-free | 0.26130 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.054 | 0.032 | 0.486 |
| Number of reflections | 28779 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 98.7 | 99.6 | 88.7 |
| Redundancy | 13.1 | 13.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG-3350, 0.3M Sodium malonate, 0.01M MgCl2, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
