3Q5C
Crystal structure of four-way junction with sticky end
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9922 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.640, 33.841, 40.400 |
| Unit cell angles | 112.77, 90.00, 110.44 |
Refinement procedure
| Resolution | 29.357 - 2.501 |
| R-factor | 0.2425 |
| Rwork | 0.241 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.739 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.278 |
| Number of reflections | 3490 | |
| <I/σ(I)> | 3.8 | 1.4 |
| Completeness [%] | 94.1 | 93.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1mM DNA, 50mM Cacodylate buffer, 50mM CaCl2, 1mM spermine, 50% MPD in Reservior, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
