3Q58
Structure of N-acetylmannosamine-6-Phosphate Epimerase from Salmonella enterica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-08-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.77483 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.153, 139.310, 38.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.430 - 1.800 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.519 |
Number of reflections | 39170 | |
<I/σ(I)> | 23.4 | 3.5 |
Completeness [%] | 92.5 | 84.6 |
Redundancy | 7.6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 200mM sodium chloride, 25% PEG3350, 100mM BisTris, 2% isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |