3Q58
Structure of N-acetylmannosamine-6-Phosphate Epimerase from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-08-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.77483 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.153, 139.310, 38.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.430 - 1.800 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.519 |
| Number of reflections | 39170 | |
| <I/σ(I)> | 23.4 | 3.5 |
| Completeness [%] | 92.5 | 84.6 |
| Redundancy | 7.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 200mM sodium chloride, 25% PEG3350, 100mM BisTris, 2% isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
