3Q54
Crystal structure of Escherichia coli BamB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-30 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 49.150, 121.123, 133.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.196 - 2.004 |
R-factor | 0.1754 |
Rwork | 0.172 |
R-free | 0.23160 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.071 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 78.2 | 60.2 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Lithium sulfate monohydrate 0.1M BIS-TRIS, pH 6.5, 25%PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |