3Q4N
Crystal structure of hypothetical protein MJ0754 from Methanococcus jannaschii DSM 2661
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 |
| Unit cell lengths | 127.015, 127.015, 48.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.750 - 2.880 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.25740 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.980 |
| High resolution limit [Å] | 2.880 | 6.200 | 2.880 |
| Rmerge | 0.147 | 0.110 | 0.413 |
| Number of reflections | 9543 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 91.8 | 99.7 | 50.2 |
| Redundancy | 19.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M Bis-Tris, 22% PEG 3350, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
