3Q49
Crystal structure of the TPR domain of CHIP complexed with Hsp70-C peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 46.033, 77.967, 37.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.678 - 1.543 |
R-factor | 0.1695 |
Rwork | 0.168 |
R-free | 0.20650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c2l |
RMSD bond length | 0.005 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.062 | 0.195 |
Number of reflections | 20035 | |
<I/σ(I)> | 52.2 | 11.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.5 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100mM Bis-tris propane pH 7.0, 40% PEG MME 2000, 3% (w/v) xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |