3Q47
Crystal structure of TPR domain of CHIP complexed with pseudophosphorylated Smad1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.010, 77.411, 36.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.700 - 1.705 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.727 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.265 |
| Number of reflections | 14183 | |
| <I/σ(I)> | 41.1 | 6.9 |
| Completeness [%] | 97.0 | 82.4 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100mM Bis-tris propane pH 7.0, 41% PEG MME 2000, 50mM magnesium acetate, 50mM proline, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
