3Q41
Crystal structure of the GluN1 N-terminal domain (NTD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-04-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 164.679, 164.679, 147.258 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.240 - 3.400 |
R-factor | 0.23185 |
Rwork | 0.229 |
R-free | 0.27929 |
Structure solution method | SAD,SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.600 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.150 | 0.630 |
Number of reflections | 32023 | |
<I/σ(I)> | 12.9 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | protein in (20mM Tris-HCl pH 7.5, 150mM NaCl) was mixed 1:1 with 4M Formate and 20mM TCEP, temperature 277K, VAPOR DIFFUSION, HANGING DROP |