3Q3U
Trametes cervina lignin peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.904, 72.931, 95.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.900 - 1.850 |
| R-factor | 0.1511 |
| Rwork | 0.149 |
| R-free | 0.19564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fm4 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.839 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.216 |
| Number of reflections | 26056 | |
| <I/σ(I)> | 24.1 | 9.1 |
| Completeness [%] | 96.6 | 77.2 |
| Redundancy | 4.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 30% MPD, 10% PEG 4000, 0.1M Imidazole-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
