3Q3Q

Crystal Structure of SPAP: an novel alkaline phosphatase from bacterium Sphingomonas sp. strain BSAR-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Detector technology	CCD
Collection date	2009-03-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.980159
Spacegroup name	P 41 21 2
Unit cell lengths	87.370, 87.370, 168.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.785 - 1.953
R-factor	0.1569
Rwork	0.155
R-free	0.18630
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.019
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.785
High resolution limit [Å]	1.950
Number of reflections	87058

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M Ammonium Sulfate, 10% Dioxane, 100mM MES Buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M Ammonium Sulfate, 10% Dioxane, 100mM MES Buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K