3Q3H
Crystal structure of the Actinobacillus pleuropneumoniae HMW1C glycosyltransferase in complex with UDP-GLC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-16 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.245, 94.896, 176.791 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.810 - 2.250 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24400 |
Structure solution method | SIR |
RMSD bond length | 0.019 |
RMSD bond angle | 1.812 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.084 | 0.271 |
Number of reflections | 61133 | |
Completeness [%] | 92.8 | 74.2 |
Redundancy | 3.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1 M MES, 0.1-0.16 M ammonium sulfate, 20-30% w/v PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |