3Q3D
Crystal structure of BmrR bound to puromycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-24 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 106.486, 106.486, 145.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.840 - 2.790 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.816 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.841 | 2.860 |
High resolution limit [Å] | 2.785 | 2.790 |
Number of reflections | 17912 | |
Completeness [%] | 83.0 | 38.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 291 | 1.0 M Sodium Malonate ) 0.05% jeffamine-M600 pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |