3Q34
The crystal structure of YceI-like family protein from Pseudomonas syringae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.647, 103.357, 63.193 |
Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
Resolution | 47.481 - 1.700 |
R-factor | 0.1722 |
Rwork | 0.170 |
R-free | 0.20670 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.697 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.700 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.075 | 0.617 |
Number of reflections | 72488 | |
<I/σ(I)> | 17.8 | 2.9 |
Completeness [%] | 99.6 | 98.2 |
Redundancy | 7.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |