3Q33
Structure of the Rtt109-AcCoA/Vps75 Complex and Implications for Chaperone-Mediated Histone Acetylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.543, 119.570, 80.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.160 - 2.800 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3D35 3DM7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX (phenix.refine: 1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 23924 | |
<I/σ(I)> | 26.1 | 2.4 |
Completeness [%] | 94.9 | 78.1 |
Redundancy | 4.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 10.0% (v/v) PEG 8000 8% (v/v) ethylene glycol 100 mM Hepes, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.5 |