3Q2U
Structure of Human Glioma Pathogenesis-related Protein 1 Reveals Unique loops and surface motifs.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-12 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.514 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.077, 79.532, 38.849 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.790 - 1.850 |
R-factor | 0.13662 |
Rwork | 0.134 |
R-free | 0.18421 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q2r |
RMSD bond length | 0.026 |
RMSD bond angle | 1.888 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.040 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 23229 | |
<I/σ(I)> | 3.2 | |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.085M MES, 25.5% w/v PEG 5000 MME, 0.17M Ammonium sulfate 15% v/v glycerol, pH 6.5, VAPOR DIFFUSION, temperature 293K |