3Q29

Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97938
Spacegroup name	P 21 21 21
格子定数 [Å]	68.390, 117.470, 146.490
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	34.532 - 2.300
R因子	0.1945
Rwork	0.193
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
結合長の平均二乗偏差(RMSD) [Å]	0.004
結合角の平均二乗偏差(RMSD) [度]	0.837
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.4_486)

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	34.532		2.360
分解能 [Å] (高)	2.300	10.290	2.300
Rmerge_l_obs	0.059	0.031	0.461
独立反射数	53017	604	3883
<I/σ(I)>	16.32	33.2	3.3
完全性 [%]	99.6	87	100

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1M TRIS pH 8.0, 2.4 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 290K