3Q27
Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.511, 49.951, 59.342 |
Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
Resolution | 59.299 - 1.302 |
R-factor | 0.1323 |
Rwork | 0.131 |
R-free | 0.15590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.164 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.000 | 80.000 | 1.350 |
High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
Rmerge | 0.078 | 0.050 | 0.568 |
Number of reflections | 82058 | ||
<I/σ(I)> | 16 | ||
Completeness [%] | 99.6 | 96.1 | 99.9 |
Redundancy | 6.2 | 6.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 290 | 0.1M bicine pH 9.0, 2.4 M ammonium sulfate, vapor diffusion, hanging drop, temperature 290K |