3Q1X
Crystal structure of Entamoeba histolytica serine acetyltransferase 1 in complex with L-serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.190, 110.190, 63.327 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.550 - 1.590 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p47 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.135 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.590 |
| Rmerge | 0.078 |
| Number of reflections | 38478 |
| Redundancy | 6.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 299 | Ammonium Sulfate, NaCl, Tris, Glycerol, L-serine, pH 8.0, vapor diffusion, hanging drop, temperature 299K |
