3Q1C

Structure of EspG Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-16
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	71.150, 74.378, 93.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.093 - 1.596
R-factor	0.1622
Rwork	0.161
R-free	0.18810
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.958	50.000	1.660
High resolution limit [Å]	1.595	3.450	1.600
Rmerge	0.035
Number of reflections	60098
<I/σ(I)>	32
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			2.0-11.0% PEG8000, 100 mM Bis-Tris, pH 6.25-7.5, VAPOR DIFFUSION, HANGING DROP, temperature range 268-291K