3Q17

Structure of a slow CLC Cl-/H+ antiporter from a cyanobacterium in Bromide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-30
Detector	ADSC QUANTUM 315
Spacegroup name	P 3 2 1
Unit cell lengths	207.209, 207.209, 105.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.479 - 3.600
R-factor	0.2819
Rwork	0.281
R-free	0.31020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nd0
RMSD bond length	0.003
RMSD bond angle	0.708
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.479	74.085	3.790
High resolution limit [Å]	3.600	11.380	3.600
Rmerge		0.016	0.016
Number of reflections	29893
<I/σ(I)>	8.7	3	0.5
Completeness [%]	97.8	99.7	96.9
Redundancy	16.8	19.6	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	30% PEG400, 0.1 M Tris-HBr, 0.2 M CaCl2 with 14 mM C-Hega-11, 6.2% PEG400 added to protein, pH 8.0, VAPOR DIFFUSION, temperature 291K