3Q16

Linkage between the Bacterial Acid Stress and Stringent Responses: The Structure of the Inducible Lysine Decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-12
Detector	MAR CCD 130 mm
Spacegroup name	C 1 2 1
Unit cell lengths	270.290, 181.290, 169.930
Unit cell angles	90.00, 125.06, 90.00

Refinement procedure

Resolution	68.000 - 4.100
R-factor	0.27771
Rwork	0.276
R-free	0.30432
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n75
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.227	68.359	4.320
High resolution limit [Å]	4.100	12.970	4.100
Rmerge	0.219	0.108	0.421
Number of reflections	40852
<I/σ(I)>	6.2	4.8	1.7
Completeness [%]	77.4	71.7	79.3
Redundancy	2.7	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	285	0.1 M Tris, 0.1 M ammonium bromide, 25-35% PEG1000, 0-10% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K