3Q15
Crystal Structure of RapH complexed with Spo0F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.064, 157.452, 84.715 |
| Unit cell angles | 90.00, 110.57, 90.00 |
Refinement procedure
| Resolution | 31.246 - 2.192 |
| R-factor | 0.2234 |
| Rwork | 0.220 |
| R-free | 0.26830 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.548 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 58465 | |
| Redundancy | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% [w/v] PEG 3350, 200 mM lithium sulphate, and 100 mM Bis-Tris (pH 6.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
