3Q0Y
N-terminal domain of C. reinhardtii SAS-6 homolog Bld12p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | MAR225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.650, 95.430, 92.010 |
| Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
| Resolution | 19.918 - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 95.550 | 2.200 | |
| High resolution limit [Å] | 2.100 | 4.000 | 2.100 |
| Rmerge | 0.109 | 0.037 | 0.589 |
| Number of reflections | 82040 | 11930 | 10636 |
| <I/σ(I)> | 11.62 | 31.7 | 2.6 |
| Completeness [%] | 99.7 | 98.8 | 99.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 20% PEG 4000, 100 MM HEPES, PH 7.0, VAPOR DIFFUSION, temperature 298K |
