3Q0Q
Crystal structure of the PUMILIO-homology domain from Human PUMILIO2 in complex with p38alpha NREa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.438, 43.030, 60.845 |
| Unit cell angles | 71.61, 85.73, 77.99 |
Refinement procedure
| Resolution | 24.061 - 2.000 |
| R-factor | 0.1969 |
| Rwork | 0.194 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.4_6)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.100 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 21704 | |
| Completeness [%] | 93.1 | 77.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 17-20% (w/v) PEG 3000, 100 mM Na3Citrate pH 5.0-6.0, VAPOR DIFFUSION, temperature 293K |
