3Q0K

Crystal structure of Human PACSIN 2 F-BAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	31.562, 353.588, 86.045
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	29.630 - 2.600
R-factor	0.206
Rwork	0.201
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3haj
RMSD bond length	0.014
RMSD bond angle	1.601
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	44004
Completeness [%]	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	288	100mM MgCl2, 100mM NaCacodylate pH 6.5, 18% PEG 3350 (w/v), VAPOR DIFFUSION, SITTING DROP, temperature 288K