3Q0I

Methionyl-tRNA formyltransferase from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	66.692, 66.692, 183.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.300 - 1.890
R-factor	0.1774
Rwork	0.176
R-free	0.20290
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.710
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.300	50.000	1.920
High resolution limit [Å]	1.890	5.130	1.890
Rmerge	0.077	0.058	0.807
Number of reflections	34252
<I/σ(I)>	14.2		3.26
Completeness [%]	100.0	99.3	100
Redundancy	12.9	12.3	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M potassium sulfate, 20 % PEG-3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K