3Q0F

Crystal structure of SUVH5 SRA- methylated CHH DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	102.968, 102.968, 170.493
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.283 - 2.750
R-factor	0.2315
Rwork	0.229
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SRA molecule from the SUVH5 SRA-fully methylated CG DNA crystallized in P42212 space group
RMSD bond length	0.007
RMSD bond angle	1.294
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (MR)
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.090	0.700
Number of reflections	14578
<I/σ(I)>	26	2.2
Completeness [%]	99.8	100
Redundancy	8.5	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5		18% PEG4000, 100 mM Na-cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP