3Q0D

Crystal structure of SUVH5 SRA- hemi methylated CG DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 42
Unit cell lengths	76.379, 76.379, 74.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.095 - 2.370
R-factor	0.2265
Rwork	0.225
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SRA Molecule from the SUVH5 SRA-fully methylated Cg DNA crystallized in space group P42212
RMSD bond length	0.009
RMSD bond angle	1.378
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (MR)
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.370
Rmerge	0.070
Number of reflections	17234
<I/σ(I)>	30.1
Completeness [%]	98.8
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	19% PEG4000, 100 mM MES, pH 6.5, 200 mM NaCl and 3 mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 291K