3Q0C
Crystal structure of SUVH5 SRA-fully methylated CG DNA complex in space group P6122
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.462, 104.462, 176.691 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.847 - 2.657 |
| R-factor | 0.2532 |
| Rwork | 0.251 |
| R-free | 0.30340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (MR) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.136 | 0.830 |
| Number of reflections | 17149 | |
| <I/σ(I)> | 27.1 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 28.1 | 28.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 23% PEG 3350, 100 mM Bis-Trispropane, pH 7.0, 80 mM Mg-acetate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
