3Q0A
X-ray crystal structure of the transcription initiation complex of the N4 mini-vRNAP with P2 promoter: Mismatch complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.346, 111.379, 275.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.690 |
| R-factor | 0.21986 |
| Rwork | 0.217 |
| R-free | 0.27595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 68042 | |
| Completeness [%] | 96.1 | 63.5 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 20% PEG 3350, 0.1 M Tris-HCl, 0.2 M dibasic ammonium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | dibasic ammonium citrate | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | Tris-HCl | ||
| 6 | 1 | 2 | dibasic ammonium citrate |
