Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.704, 202.850, 247.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.910 - 3.050 |
| R-factor | 0.18037 |
| Rwork | 0.178 |
| R-free | 0.22079 |
| Structure solution method | Phaser |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.723 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.105 | 0.348 |
| Number of reflections | 117263 | |
| <I/σ(I)> | 4.682 | |
| Completeness [%] | 99.3 | 97.2 |
| Redundancy | 7.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 16% PEG 8000, 200mM CaAc2, 25mM NaNO2, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298 K, temperature 298.0K |
