3PZF
1.75A resolution structure of Serpin-2 from Anopheles gambiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-06 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 63 |
Unit cell lengths | 96.538, 96.538, 78.289 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.254 - 1.750 |
R-factor | 0.1751 |
Rwork | 0.173 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Serpin-2 model from lower resolution data that was subsequently used for molecular replacement against this higher resolution data |
RMSD bond length | 0.011 |
RMSD bond angle | 1.233 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.2.3) |
Refinement software | PHENIX (dev_605) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.059 | 0.415 |
Number of reflections | 41856 | |
<I/σ(I)> | 35.946 | 2.32 |
Completeness [%] | 96.5 | 78.7 |
Redundancy | 11.1 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 2.8 M Sodium Acetate, pH 7.0, vapor diffusion, temperature 293K |