3PZ7
Crystal structure of Ccd1-DIX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.576, 62.576, 77.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.581 - 2.441 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.27510 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.581 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.110 | 0.608 |
| Number of reflections | 3659 | |
| <I/σ(I)> | 18.6 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 0.1M Sodium Cacodylate, 20% PEG 3350, 4% Ethylene Glycol, 0.2M Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K |
