3PYP
PHOTOACTIVE YELLOW PROTEIN, CRYOTRAPPED EARLY LIGHT CYCLE INTERMEDIATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 149 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.224, 66.224, 40.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 0.850 |
| R-factor | 0.1326 |
| R-free | 0.15480 |
| Structure solution method | SAME CRYSTAL LATTICE MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELX-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 0.860 | |
| High resolution limit [Å] | 0.850 | 0.850 |
| Rmerge | 0.042 | 0.520 |
| Total number of observations | 561693 * | |
| Number of reflections | 84934 | |
| <I/σ(I)> | 10.9 | 1.7 |
| Completeness [%] | 97.7 | 87.1 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | used as seeds * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 2 | 1 | reservoir | sodium phosphate | 20 (mM) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | drop | ammonium sulfate | 2.5 (M) | |
| 5 | 1 | drop | sodium phosphate | 20 (mM) |
