3PYI
Structure of the N-terminal domain of C. elegans SAS-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.273, 73.153, 79.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.678 - 2.104 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.25740 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.153 | 2.220 |
| High resolution limit [Å] | 2.104 | 2.104 |
| Rmerge | 0.039 | 0.319 |
| Number of reflections | 24368 | |
| <I/σ(I)> | 17.7 | 4 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 30% v/v PEG200, 5% w/v PEG3000, 0.1 M MES, seeding with crushed original crystals, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
