3PY9
X-ray structural studies of the entire extra-cellular region of the Ser/Thr kinase PrkC from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-30 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 55.681, 69.103, 88.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.970 - 2.200 |
R-factor | 0.24674 |
Rwork | 0.244 |
R-free | 0.29346 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.286 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | |
Number of reflections | 18045 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 5 mg/mL protein solution, 21% (w/v) MPEG2000, 3% w/v D-(+)-galactose, 160 mM ammonium sulfate and 60 mM sodium acetate trihydrate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |