3PXL
Type-2 Cu-depleted fungus laccase from Trametes hirsuta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8156 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.653, 77.051, 130.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 1.200 |
R-factor | 0.15397 |
Rwork | 0.153 |
R-free | 0.17903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fpx |
RMSD bond length | 0.016 |
RMSD bond angle | 1.687 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 155833 | |
Completeness [%] | 97.0 | 97 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Protein solution 44 mg/ml in 0.02M potassium phosphate, pH 6.0. Reservoir solution is 30% PEG 1500, 0.02M potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |