3PXJ

Tandem Ig repeats of Dlar

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	72.957, 77.513, 81.726
Unit cell angles	90.00, 101.08, 90.00

Refinement procedure

Resolution	41.385 - 2.300
R-factor	0.2251
Rwork	0.223
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pxh
RMSD bond length	0.032
RMSD bond angle	1.657
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.117	0.108	0.415
Number of reflections	39287
<I/σ(I)>	12.3
Completeness [%]	98.7	95.7	98.2
Redundancy	2.7	2.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	50 mM imidazole pH 7.0, 10 mM ammonium sulfate, 20% (w/v) PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K