3PXH
Tandem Ig domains of tyrosine phosphatase LAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.450, 77.450, 68.499 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.537 - 2.001 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES IFHG AND 2IEP |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.674 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.069 | 0.045 | 0.364 |
| Number of reflections | 15225 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 92.6 | 99.1 | 65.4 |
| Redundancy | 15.4 | 17 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% (w/v) PEG 1500, 50 mM citrate 5.5, 200 mM lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
