3PWZ
Crystal structure of an Ael1 enzyme from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-10 |
| Detector | SBC-3 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.281, 49.246, 60.510 |
| Unit cell angles | 90.00, 114.40, 90.00 |
Refinement procedure
| Resolution | 30.147 - 1.705 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.081 | 0.105 |
| Number of reflections | 27408 | |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 90.0 | 57 |
| Redundancy | 4.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 0.1M NaHEPES, 2M (NH4)2SO4, and 1.5% PEG400, pH 7.5, temperature 298K |
