3PWX

Structure of putative flagellar hook-associated protein from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	100.687, 63.509, 80.570
Unit cell angles	90.00, 107.86, 90.00

Refinement procedure

Resolution	35.980 - 2.500
R-factor	0.2335
Rwork	0.230
R-free	0.29430
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.731
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.074	0.049	0.461
Number of reflections	16729
<I/σ(I)>	21.1
Completeness [%]	99.8	98.7	98.4
Redundancy	3.7	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1M Tris pH8.5, 30% PEG 4K, 0.2M Magnesium Chloride hexahydrate, pH 5.5, Vapor diffusion, Sitting drop, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1M Tris pH8.5, 30% PEG 4K, 0.2M Magnesium Chloride hexahydrate, pH 5.5, Vapor diffusion, Sitting drop, temperature 298K