3PWU
An immmunodominant CTL epitope from rinderpest virus presented by cattle MHC class I molecule N*01801(BoLA-A11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-28 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.913, 72.044, 120.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.956 - 1.899 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e27 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.909 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.899 | 1.900 |
| Rmerge | 0.066 | 0.535 |
| Number of reflections | 32536 | |
| <I/σ(I)> | 29.174 | 3.138 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
