3PW9
Structural and functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97622 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.147, 49.762, 76.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.500 - 2.100 |
Rwork | 0.189 |
R-free | 0.24800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3pvh |
RMSD bond angle | 0.012 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.063 | 0.036 | 0.496 |
Number of reflections | 10968 | ||
<I/σ(I)> | 26.4 | 36.3 | 3.7 |
Completeness [%] | 99.5 | 96.8 | 99.9 |
Redundancy | 5.3 | 4.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate (pH 6.5), 25-30% (w/v) Polyethylene glycol 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 296K |