3PVL
Structure of myosin VIIa MyTH4-FERM-SH3 in complex with the CEN1 of Sans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 98.864, 98.864, 242.278 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.22922 |
Rwork | 0.228 |
R-free | 0.25903 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.874 |
High resolution limit [Å] | 2.800 | 2.801 |
Rmerge | 0.060 | 0.060 |
Number of reflections | 32761 | |
<I/σ(I)> | 31 | 31 |
Completeness [%] | 99.6 | 99.69 |
Redundancy | 9.1 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.02M MgCl2, 0.1M HEPES, pH 7.5, 22% (w/v) polyacrylic acid sodium salt 5100, VAPOR DIFFUSION, HANGING DROP, temperature 289K |