3PVC
Crystal structure of apo MnmC from Yersinia Pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.146, 59.566, 99.630 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.17282 |
| Rwork | 0.170 |
| R-free | 0.23089 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.475 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.118 | 0.689 |
| Number of reflections | 33290 | |
| <I/σ(I)> | 14.8 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES PH 7.0 30% v/v Jeffamine ED-2001 Reagent PH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
