3PV7
Crystal structure of NKp30 ligand B7-H6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.502 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.300, 70.600, 53.200 |
| Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.246 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.749 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.067 | 0.045 | 0.604 |
| Number of reflections | 21807 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 99.9 | 99.1 | 99.6 |
| Redundancy | 7.3 | 7.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | PEG 3350, LiCl2, pH 7.0, hanging drop, temperature 298K |
