3PV6
Crystal structure of NKp30 bound to its ligand B7-H6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.502 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.874, 74.868, 125.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.239 |
R-free | 0.28870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.988 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 21751 | |
<I/σ(I)> | 53 | 3.3 |
Completeness [%] | 98.4 | 88.2 |
Redundancy | 14 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 298 | PEG 3350, KF, pH 7.0, hanging drop, temperature 298K |