3PUY
Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to AMP-PNP after crystal soaking of the pretranslocation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03324 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.550, 96.283, 112.283 |
| Unit cell angles | 86.91, 80.87, 74.32 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.21629 |
| Rwork | 0.214 |
| R-free | 0.25427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.061 | |
| Number of reflections | 46613 | |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 64.9 | 23.9 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 32% PEG 400, 0.1 M HEPES, 40 mM magnesium chloride, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |
